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Now that you have this, you pour a small amount of solvent.
And as it does that, it'll actually take some of the dyes from that green spot with it.
And let's see what happens at a few different time points.
The main difference is that instead of having a piece of paper, you have a glass slide that is coated with a layer of silica gel.
This is a great preparative tool that is commonly used in the organic chemistry lab.
Next, you'll want to prep a beaker, or actually, any kind of container will do.
But in this, you'll be putting in your mobile phase. It could be water, or it could be any organic solvent you want.The next kind of chromatography that's almost identical to paper chromatography is known as thin-layer chromatography, or TLC for short.We'll see that all the spots on this table are pretty much the same.Have you ever scribbled something down with a black pen on a piece of paper, then accidentally smudged the paper with water? Well, what happens is that you might see the black ink start to smudge and start to see some colors that aren't black at all, maybe some dark blues and some darker purples. This is because black dye is actually made out of a bunch of different other dyes and different components.And what you've just witnessed is a basic example of paper chromatography.You'll see that the spots will continue traveling even farther up the plate, and the separation between them, that distance will increase even more. Whereas the yellow spot didn't move quite as much, which means it was more attracted to the paper for the stationary phase.So ultimately what you've shown here is that whatever was in the green spot originally wasn't just one compound. This competition between the stationary phase and the mobile phase pulling at the components is what drives the separation that occurs in all different kinds of chromatography.So let's try to lay this information out in a table.We've talked about how for paper chromatography, the stationary phase is a solid; the mobile phase is some kind of solvent, so a liquid; and they're separating it based on polarity, meaning how attracted it is to the paper versus the solvent, depending on its chemical properties.If you're seeing this message, it means we're having trouble loading external resources on our website.If you're behind a web filter, please make sure that the domains *.and *.are unblocked.