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In addition, the biosurfactant showed emulsifying activity against the following hydrocarbons: petrol, kerosene, xylene, toluene, and diesel.The optimum cultural conditions (temperature, p H, carbon, nitrogen, hydrocarbon, inoculum concentration, and incubation time) for growth and biosurfactant production by K. The biosurfactant was characterized as a phospholipid using TLC, while the GC–MS analysis identified the phospholipid as phosphatidylethanolamine. pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil to produce biosurfactant and the effectiveness of the produced biosurfactant in emulsifying different hydrocarbons.One application of biosurfactant that is of interest to environmentalist is in environmental management and bioremediation.
For each soil source, soil samples were randomly collected from different points at depths between 0 and 15 cm using a hand-held soil auger and then bulked to get a composite sample.
The samples were transported aseptically in sterile polythene bags to the laboratory for the analysis.
The biosurfactant screening techniques employed were emulsification assay, emulsification index (E ), lipase activity, haemolytic assay, oil spreading, and tilted glass slide.
The bacterial isolate was identified based on phenotypic, biochemical, and molecular means.
The phylogenetic tree analysis of the 16S r RNA gene classified the isolate as K. The sequence obtained from the isolate has been deposited in Gen Bank under the accession number KT254060.1.
The result obtained from the study revealed high biosurfactant activity with a maximum E of 70 % by sodium dodecyl sulphate (SDS).
Considerable attention has been given to the use of biosurfactants in recent times because of their potential industrial and environmental applications and ecological friendliness.
Hydrocarbon-polluted soils have been major sources of biosurfactant-producing bacteria; resultantly, this study had been aimed at isolating and characterizing biosurfactant produced by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria.
It has been reported that 2–3 % of screened populations in uncontaminated soils are biosurfactant-producing microorganisms.
This figure increases to 25 % in polluted soils (Bodour et al. On the other hand, enrichment culture techniques specific for hydrocarbon-degrading bacteria may lead to a much higher detection of biosurfactant producers with estimates up to 80 % (Rahman et al. Biosurfactants produced by microorganisms are grouped into two different classes based on their chemical composition, viz., low molecular weight surface-active agents called biosurfactants and high molecular weight biosurfactants referred to as bioemulsifiers.